2024
- A single-cell atlas of the aging mouse ovary. Nat Aging. 2024 Jan;4(1):145-162.
- Reproductive Ageing: Inflammation, immune cells, and cellular senescence in the aging ovary. Isola JVV, Hense JD, Osório CAP, Biswas S, Alberola-Ila J, Ocañas SR, Schneider A, Stout MB. 2024 Jun 21;168(2):e230499
- Age- and sex- divergent translatomic responses of the mouse retinal pigmented epithelium. Chucair-Elliott AJ, Ocañas SR, Pham K, Machalinski A, Plafker S, Stout MB, Elliott MH, Freeman WM. Neurobiol Aging. 2024 Aug;140:41-59.
- Flow Cytometry Analysis of Microglial Phenotypes in the Murine Brain During Aging and Disease. Cox JEJ, Pham KD, Keck AW, Wright Z, Thomas MA, Freeman WM, Ocañas SR. Bio Protoc. 2024 Jun 20;14(12):e5018
- Microglia undergo sex-dimorphic transcriptional and metabolic rewiring during aging. Kang S, Ko EY, Andrews AE, Shin JE, Nance KJ, Barman PK, Heeger PS, Freeman WM, Benayoun BA, Goodridge HS. J Neuroinflammation. 2024 Jun 5;21(1):150.
- Age- and sex- divergent translatomic responses of the mouse retinal pigmented epithelium. Chucair-Elliott AJ, Ocañas SR, Pham K, Machalinski A, Plafker S, Stout MB, Elliott MH, Freeman WM. Neurobiol Aging. 2024 Aug;140:41-59
- Transcriptomic Analysis Reveals That Excessive Thyroid Hormone Signaling Impairs Phototransduction and Mitochondrial Bioenergetics and Induces Cellular Stress in Mouse Cone Photoreceptors. Ma H, Stanford D, Freeman WM, Ding XQ. Int J Mol Sci. 2024 Jul 6;25(13):7435
2023
- Specificity and efficiency of tamoxifen-mediated Cre induction is equivalent regardless of age. iScience. 2023 Nov 9;26(12):108413.
- Characterization of novel mouse models to study the role of necroptosis in aging and age-related diseases. Geroscience. 2023 Dec;45(6):3241-3256.
- Microglial senescence contributes to female-biased neuroinflammation in the aging mouse hippocampus: implications for Alzheimer’s disease. J Neuroinflammation. 2023 Aug 16;20(1):188
- Heterochronic Plasma Transfer: Experimental Design, Considerations, and Technical Challenges. Rejuvenation Res. 2023 Oct;26(5):171-179.
- Differential usage of DNA modifications in neurons, astrocytes, and microglia. Epigenetics Chromatin. 2023 Nov 13;16(1):45.
- Microglial MHC-I induction with aging and Alzheimer’s is conserved in mouse models and humans. 2023 Oct;45(5):3019-3043.
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Absence of either Ripk3 or Mlkl reduces incidence of hepatocellular carcinoma independent of liver fibrosis. Mol Cancer Res. 2023 Sep 1;21(9):933-946.
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Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome. J Vis Exp. 2023 Feb 24;(192):10.3791/64765.
- Assessing tolerability and physiological responses to 17α-estradiol administration in male rhesus macaques. Geroscience. 2023 Aug;45(4):2337-2349.
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Chromosomal and gonadal factors regulate microglial sex effects in the aging brain. Brain Res Bull. 2023 Apr;195:157-171
- Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation. Methods Mol Biol. 2023;2625:7-15.
- 17α-estradiol, a lifespan-extending compound, attenuates liver fibrosis by modulating collagen turnover rates in male mice. Am J Physiol Endocrinol Metab. 2023 Feb 1;324(2):E120-E134
2022
- Translatomic response of retinal Müller glia to acute and chronic stress. Neurobiol Dis. 2022 Dec;175:105931.
- An RNA-seq study of the mPFC of rats with different addiction phenotypes. Brain Res Bull. 2022 Dec;191:107-120.
- Differential Regulation of Mouse Hippocampal Gene Expression Sex Differences by Chromosomal Content and Gonadal Sex. Mol Neurobiol. 2022 Aug;59(8):4669-4702.
- Scavenging mitochondrial hydrogen peroxide by peroxiredoxin 3 overexpression attenuates contractile dysfunction and muscle atrophy in a murine model of accelerated sarcopenia. Ahn B, Ranjit R, Kneis P, Xu H, Piekarz KM, Freeman WM, Kinter M, Richardson A, Ran Q, Brooks SV, Van Remmen H. Aging Cell. 2022 Feb 24:e13569.
- Minimizing the Ex Vivo Confounds of Cell-Isolation Techniques on Transcriptomic and Translatomic Profiles of Purified Microglia. eNeuro. 2022 Mar 28;9(2):ENEURO.0348-21.2022.
2021
- The NOTCH3 Downstream Target HEYL is Required for Efficient Human Airway Basal Cell Differentiation. Bodas M, Subramaniyan B, Moore AR, Metcalf JP, Ocañas SR, Freeman WM, Georgescu C, Wren JD, Walters MS. Cells 2021 Nov 18;10(11):3215.
- Repeated cocaine or methamphetamine treatment alters astrocytic CRF2 and GLAST expression in the ventral midbrain. Sharpe AL, Trzeciak M, Eliason NL, Blankenship H, Byrd B, Douglas P, Freeman WM, Beckstead MJ. Addiction Biology, 2021 Nov 25:e13120.
- Many chronological aging clocks can be found throughout the epigenome: Implications for quantifying biological aging. Porter HL, Brown CA, Roopnarinesingh X, Giles CB, Georgescu C, Freeman WM, Wren JD. Aging Cell. Aging Cell 2021 Oct 16:e13492.
- Oklahoma Nathan Shock Aging Center – assessing the basic biology of aging from genetics to protein and function. Van Remmen H, Freeman WM, Miller BF, Kinter M, Wren JD, Chiao A, Towner RA, Snider TA, Sonntag WE, Richardson A. Geroscience 2021 Oct;43(5):2183-2203
- Litter expansion alters metabolic homeostasis in a sex specific manner. Kurup K, Mann SN, Jackson J, Matyi S, Ranjo-Bishop M, Freeman WM, Stout MB, Richardson A, Unnikrishnan A. PLoS One. 2021 Sep 29;16(9):e0237199.
- Long-term, induced expression of Hand2 in peripheral sympathetic neurons ameliorates sarcopenia in geriatric mice. Rodrigues ACZ, Messi ML, Wang ZM, Bonilla HJ, Freeman WM, Delbono O. J Cachexia Sarcopenia Muscle. J Cachexia Sarcopenia Muscle. 2021 Dec;12(6):1908-1924.
- Cigarette Smoke Activates NOTCH3 to Promote Goblet Cell Differentiation in Human Airway Epithelial Cells. Am J Respir Cell Mol Biol. 2021 Apr;64(4):426-440. doi: 10.1165/rcmb.2020-0302OC.
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Cellular hallmarks of aging emerge in the ovary prior to primordial follicle depletion. Ansere VA, Ali-Mondal S, Sathiaseelan R, Garcia DN, Isola JVV, Henseb JD, Saccon TD, Ocañas SR, Tooley KB, Stout MB, Schneider A, Freeman WM. Mech Ageing Dev. 2021 Mar;194:111425. doi: 10.1016/j.mad.2020.111425.
2020
- Inducible cell-specific mouse models for paired epigenetic and transcriptomic studies of microglia and astroglia. Chucair-Elliott AJ, Ocanas SR, Stanford DR, Ansere VA, Buettner KB, Porter H, Eliason NL, Reid J, Sharpe AL, Stout MB, Beckstead MJ, Miller BF, Richardson A, Freeman WM. Communications Biology. 2020 3:693.
- Exercising your mind. Ansere VA and Freeman WM. Science 2020 369:144-145. doi: 10.1126/science.abc8830. Full Text
- Health benefits attributed to 17α-estradiol, a lifespan-extending compound, are mediated through estrogen receptor α. Mann SN, Hadad N, Nelson Holte M, Rothman AR, Sathiaseelan R, Ali Mondal S, Agbaga MP, Unnikrishnan A, Subramaniam M, Hawse J, Huffman DM, Freeman WM, Stout MB. Elife. 2020 Dec 8;9:e59616. doi: 10.7554/eLife.59616.
- Female mice are resilient to age-related decline of substantia nigra dopamine neuron firing parameters.
Neurobiol Aging. 2020 Nov;95:195-204. doi: 10.1016/j.neurobiolaging.2020.07.025.
Heart and neural crest derivative 2-induced preservation of sympathetic neurons attenuates sarcopenia with aging. J Cachexia Sarcopenia Muscle. 2020 Nov 30. doi: 10.1002/jcsm.12644.
- Opposite Roles of the JMJD1A Interaction Partners MDFI and MDFIC in Colorectal Cancer. Sui Y, Li X, Oh S, Zhang B, Freeman WM, Shin S, Janknecht R. Sci Rep. 2020 10:8710. doi: 10.1038/s41598-020-65536-6.
- Cooperation between ETS transcription factor ETV1 and histone demethylase JMJD1A in colorectal cancer.Int J Oncol. 2020 Dec;57(6):1319-1332. doi: 10.3892/ijo.2020.5133.
- Molecular changes in transcription and metabolic pathways underlying muscle atrophy in the CuZnSOD null mouse model of sarcopenia. Sataranatarajan K, Pharaoh G, Brown JL, Ranjit R, Piekarz KM, Street K, Wren JD, Georgescu C, Kinter C, Kinter M, Freeman WM, Richardson A, Van Remmen H. Geroscience. 2020 May 12. doi: 10.1007/s11357-020-00189-x.
- Short-term calorie restriction and 17α-estradiol administration elicit divergent effects on proteostasis processes and protein content in metabolically active tissues. Miller BF, Pharaoh GA, Hamilton KL, Peelor FF, Kirkland JL, Freeman WM, Mann SN, Kinter M, Price JC, Stout MB. J Geronotol A Biol Sci Med Sci. 2020 75:849-857. doi: 10.1093/gerona/glz113.
2019
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Weight Loss Results in Increased Expression of Anti-Inflammatory Protein CRISPLD2 in Mouse Adipose Tissue. Jackson RM, Griesel BA, Short KR, Sparling D, Freeman WM, Olson AL. Obesity. 2019 27:2025-2036. doi: 10.1002/oby.22652.
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Early-life DNA methylation profiles are indicative of age-related transcriptome changes. Hadad N, Masser DR, Blanco-Berdugo L, Stanford DR, Freeman WM.Epigenetics Chromatin. 2019 12:58. doi: 10.1186/s13072-019-0306-5.
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Tamoxifen induction of Cre recombinase does not cause long-lasting or sexually divergent responses in the CNS epigenome or transcriptome: implications for the design of aging studies. Chucair-Elliott AJ, Ocanas SR, Stanford DR, Hadad N, Wronowski B, Otalora L, Stout MB, Freeman WM. Geroscience. 2019 41:691-708. doi: 10.1007/s11357-019-00090-2.
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Mitochondrial oxidative stress impairs contractile function but paradoxically increases muscle mass via fibre branching. Ahn B, Ranjit R, Premkumar P, Pharaoh G, Piekarz KM, Matsuzaki S, Claflin DR, Riddle K, Judge J, Bhaskaran S, Satara Natarajan K, Barboza E, Wronowski B, Kinter M, Humphries KM, Griffin TM, Freeman WM, Richardson A, Brooks SV, Van Remmen H. J Cachexia Sarcopenia Muscle. 2019 10:411-428. doi: 10.1002/jcsm.12375.
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The role of DNA methylation in epigenetics of aging. Unnikrishnan A, Freeman WM, Jackson J, Wren JD, Porter H, Richardson A. Pharmacol Ther. 2019 195:172-185. doi: 10.1016/j.pharmthera.2018.11.001.
2018
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Age-related focal loss of contractile vascular smooth muscle cells in retinal arterioles is accelerated by caveolin-1 deficiency. Reagan AM, Gu X, Paudel S, Ashpole NM, Zalles M, Sonntag WE, Ungvari Z, Csiszar A, Otalora L, Freeman WM, Stout MB, Elliott MH. Neurobiol Aging. 2018 Jul 10;71:1-12. doi: 10.1016/j.neurobiolaging.2018.06.039.
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Exposure to environmental enrichment attenuates addiction-like behavior and alters molecular effects of heroin self-administration in rats. Imperio CG, McFalls AJ, Hadad N, Blanco-Berdugo L, Masser DR, Colechio EM, Coffey AA, Bixler GV, Stanford DR, Vrana KE, Grigson PS, Freeman WM. Neuropharmacology. 2018 Jun 28;139:26-40. doi: 10.1016/j.neuropharm.2018.06.037.
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Caloric restriction mitigates age-associated hippocampal differential CG and non-CG methylation. Hadad N, Unnikrishnan A, Jackson JA, Masser DR, Otalora L, Stanford DR, Richardson A, Freeman WM. Neurobiol Aging. 2018 Jul;67:53-66. doi: 10.1016/j.neurobiolaging.2018.03.009.
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Revisiting the genomic hypomethylation hypothesis of aging. Unnikrishnan A, Hadad N, Masser DR, Jackson J, Freeman WM, Richardson A. Ann N Y Acad Sci. 2018 Apr;1418(1):69-79. doi: 10.1111/nyas.13533.
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Expression of the purine biosynthetic enzyme phosphoribosyl formylglycinamidine synthase in neurons. Mangold CA, Yao PJ, Du M, Freeman WM, Benkovic SJ, Szpara ML. J Neurochem. 2018 Mar;144(6):723-735. doi: 10.1111/jnc.14304.
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Insulin-like growth factor receptor signaling regulates working memory, mitochondrial metabolism, and amyloid-β uptake in astrocytes. Logan S, Pharaoh GA, Marlin MC, Masser DR, Matsuzaki S, Wronowski B, Yeganeh A, Parks EE, Premkumar P, Farley JA, Owen DB, Humphries KM, Kinter M, Freeman WM, Szweda LI, Van Remmen H, Sonntag WE. Mol Metab. 2018 Mar;9:141-155. doi: 10.1016/j.molmet.2018.01.013.
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17α-estradiol acts through hypothalamic pro-opiomelanocortin expressing neurons to reduce feeding behavior. Steyn FJ, Ngo ST, Chen VP, Bailey-Downs LC, Xie TY, Ghadami M, Brimijoin S, Freeman WM, Rubinstein M, Low MJ, Stout MB. Aging Cell. 2018 Feb;17(1). doi: 10.1111/acel.12703.
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Analysis of DNA modifications in aging research. Masser DR, Hadad N, Porter H, Stout MB, Unnikrishnan A, Stanford DR, Freeman WM. Geroscience. 2018 Feb;40(1):11-29. doi: 10.1007/s11357-018-0005-3.
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A potential common role of the Jumonji C domain-containing 1A histone demethylase and chromatin remodeler ATRX in promoting colon cancer. Li X, Oh S, Song H, Shin S, Zhang B, Freeman WM, Janknecht R.Oncol Lett. 2018 Nov;16(5):6652-6662. doi: 10.3892/ol.2018.9487.
2017
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Sexually divergent DNA methylation patterns with hippocampal aging. Masser DR, Hadad N, Porter HL, Mangold CA, Unnikrishnan A, Ford MM, Giles CB, Georgescu C, Dozmorov MG, Wren JD, Richardson A, Stanford DR, Freeman WM. Aging Cell. 2017 Dec;16(6):1342-1352. doi: 10.1111/acel.12681.
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Functional changes in the neural retina occur in the absence of mitochondrial dysfunction in a rodent model of diabetic retinopathy. Masser DR, Otalora L, Clark NW, Kinter MT, Elliott MH, Freeman WM. J Neurochem. 2017 Dec;143(5):595-608. doi: 10.1111/jnc.14216
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Retinal gene expression responses to aging are sexually divergent. Du M, Mangold CA, Bixler GV, Brucklacher RM, Masser DR, Stout MB, Elliott MH, Freeman WM. Mol Vis. 2017 Oct 10;23:707-717
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Sexually divergent induction of microglial-associated neuroinflammation with hippocampal aging. Mangold CA, Wronowski B, Du M, Masser DR, Hadad N, Bixler GV, Brucklacher RM, Ford MM, Sonntag WE, Freeman WM. J Neuroinflammation. 2017 Jul 21;14(1):141. doi: 10.1186/s12974-017-0920-8.
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Role of DNA methylation in the dietary restriction mediated cellular memory. Unnikrishnan A, Jackson J, Matyi SA, Hadad N, Wronowski B, Georgescu C, Garrett KP, Wren JD, Freeman WM, Richardson A. Geroscience. 2017 Jun;39(3):331-345. doi: 10.1007/s11357-017-9976-8.
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CNS-wide Sexually Dimorphic Induction of the Major Histocompatibility Complex 1 Pathway With Aging. Mangold CA, Masser DR, Stanford DR, Bixler GV, Pisupati A, Giles CB, Wren JD, Ford MM, Sonntag WE, Freeman WM. J Gerontol A Biol Sci Med Sci. 2017 Jan;72(1):16-29.
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Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation. Hopiavuori BR, Masser DR, Wilkerson JL, Brush RS, Mandal NA, Anderson RE, Freeman WM. Methods Mol Biol. 2017;1609:33-41. doi: 10.1007/978-1-4939-6996-8_4.
2016
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Loss of the antioxidant enzyme CuZnSOD (Sod1) mimics an age-related increase in absolute mitochondrial DNA copy number in the skeletal muscle. Masser DR, Clark NW, Van Remmen H, Freeman WM. Age. 2016 Aug;38(4):323-333. doi: 10.1007/s11357-016-9930-1.
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Bisulfite oligonucleotide-capture sequencing for targeted base- and strand-specific absolute 5-methylcytosine quantitation. Masser DR, Stanford DR, Hadad N, Giles CB, Wren JD, Sonntag WE, Richardson A, Freeman WM. Age. 2016 Jun;38(3):49. doi: 10.1007/s11357-016-9914-1.
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Assessment of individual differences in the rat nucleus accumbens transcriptome following taste-heroin extended access. Imperio CG, McFalls AJ, Colechio EM, Masser DR, Vrana KE, Grigson PS, Freeman WM. Brain Res Bull. 2016 May;123:71-80. doi: 10.1016/j.brainresbull.2015.12.005.
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Reward devaluation and heroin escalation is associated with differential expression of CRF signaling genes. McFalls AJ, Imperio CG, Bixler G, Freeman WM, Grigson PS, Vrana KE. Brain Res Bull. 2016 May;123:81-93. doi: 10.1016/j.brainresbull.2015.11.009
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Recent Developments in Understanding Brain Aging: Implications for Alzheimer’s Disease and Vascular Cognitive Impairment. Deak F, Freeman WM, Ungvari Z, Csiszar A, Sonntag WE. J Gerontol A Biol Sci Med Sci. 2016 Jan;71(1):13-20. doi: 10.1093/gerona/glv206
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Absence of genomic hypomethylation or regulation of cytosine-modifying enzymes with aging in male and female mice. Hadad N, Masser DR, Logan S, Wronowski B, Mangold CA, Clark N, Otalora L, Unnikrishnan A, Ford MM, Giles CB, Wren JD, Richardson A, Sonntag WE, Stanford DR, Freeman W. Epigenetics Chromatin. 2016 Jul 13;9:30. doi: 10.1186/s13072-016-0080-6