Publications

2020

Cellular hallmarks of aging emerge in the ovary prior to primordial follicle depletion. Ansere VA, Ali-Mondal S, Sathiaseelan R, Garcia DN, Isola JVV, Henseb JD, Saccon TD, Ocañas SR, Tooley KB, Stout MB, Schneider A, Freeman WM. Mech Ageing Dev. 2020 Dec 28;194:111425
Health benefits attributed to 17α-estradiol, a lifespan-extending compound, are mediated through estrogen receptor α. Mann SN, Hadad N, Nelson Holte M, Rothman AR, Sathiaseelan R, Ali Mondal S, Agbaga MP, Unnikrishnan A, Subramaniam M, Hawse J, Huffman DM, Freeman WM, Stout MB. Elife. 2020 Dec 8;9:e59616
Inducible cell-specific mouse models for paired epigenetic and transcriptomic studies of microglia and astroglia. Chucair-Elliott AJ, Ocanas SR, Stanford DR, Ansere VA, Buettner KB, Porter H, Eliason NL, Reid J, Sharpe AL, Stout MB, Beckstead MJ, Miller BF, Richardson A, Freeman WM. Communications Biology. 2020 3:693.
Exercising your mind. Ansere VA and Freeman WM. Science 2020 369:144-145. doi: 10.1126/science.abc8830. Full Text
Health benefits attributed to 17α-estradiol, a lifespan-extending compound, are mediated through estrogen receptor α. Mann SN, Hadad N, Nelson Holte M, Rothman AR, Sathiaseelan R, Ali Mondal S, Agbaga MP, Unnikrishnan A, Subramaniam M, Hawse J, Huffman DM, Freeman WM, Stout MB. Elife. 2020 Dec 8;9:e59616. doi: 10.7554/eLife.59616.
Heart and neural crest derivative 2-induced preservation of sympathetic neurons attenuates sarcopenia with aging. Rodrigues ACZ, Wang ZM, Messi ML, Bonilla HJ, Liu L, Freeman WM, Delbono O.J Cachexia Sarcopenia Muscle. 2020 Nov 30. doi: 10.1002/jcsm.12644.
Opposite Roles of the JMJD1A Interaction Partners MDFI and MDFIC in Colorectal Cancer. Sui Y, Li X, Oh S, Zhang B, Freeman WM, Shin S, Janknecht R. Sci Rep. 2020 10:8710. doi: 10.1038/s41598-020-65536-6.
Cooperation between ETS transcription factor ETV1 and histone demethylase JMJD1A in colorectal cancer. Oh S, Song H, Freeman WM, Shin S, Janknecht R.Int J Oncol. 2020 Dec;57(6):1319-1332. doi: 10.3892/ijo.2020.5133.
Molecular changes in transcription and metabolic pathways underlying muscle atrophy in the CuZnSOD null mouse model of sarcopenia. Sataranatarajan K, Pharaoh G, Brown JL, Ranjit R, Piekarz KM, Street K, Wren JD, Georgescu C, Kinter C, Kinter M, Freeman WM, Richardson A, Van Remmen H. Geroscience. 2020 May 12. doi: 10.1007/s11357-020-00189-x.
Short-term calorie restriction and 17α-estradiol administration elicit divergent effects on proteostasis processes and protein content in metabolically active tissues. Miller BF, Pharaoh GA, Hamilton KL, Peelor FF, Kirkland JL, Freeman WM, Mann SN, Kinter M, Price JC, Stout MB. J Geronotol A Biol Sci Med Sci. 2020 75:849-857. doi: 10.1093/gerona/glz113.

2019

Weight Loss Results in Increased Expression of Anti-Inflammatory Protein CRISPLD2 in Mouse Adipose Tissue. Jackson RM, Griesel BA, Short KR, Sparling D, Freeman WM, Olson AL. Obesity. 2019 27:2025-2036. doi: 10.1002/oby.22652.
Early-life DNA methylation profiles are indicative of age-related transcriptome changes. Hadad N, Masser DR, Blanco-Berdugo L, Stanford DR, Freeman WM.Epigenetics Chromatin. 2019 12:58. doi: 10.1186/s13072-019-0306-5.
Tamoxifen induction of Cre recombinase does not cause long-lasting or sexually divergent responses in the CNS epigenome or transcriptome: implications for the design of aging studies. Chucair-Elliott AJ, Ocanas SR, Stanford DR, Hadad N, Wronowski B, Otalora L, Stout MB, Freeman WM. Geroscience. 2019 41:691-708. doi: 10.1007/s11357-019-00090-2.
Mitochondrial oxidative stress impairs contractile function but paradoxically increases muscle mass via fibre branching. Ahn B, Ranjit R, Premkumar P, Pharaoh G, Piekarz KM, Matsuzaki S, Claflin DR, Riddle K, Judge J, Bhaskaran S, Satara Natarajan K, Barboza E, Wronowski B, Kinter M, Humphries KM, Griffin TM, Freeman WM, Richardson A, Brooks SV, Van Remmen H. J Cachexia Sarcopenia Muscle. 2019 10:411-428. doi: 10.1002/jcsm.12375.
The role of DNA methylation in epigenetics of aging. Unnikrishnan A, Freeman WM, Jackson J, Wren JD, Porter H, Richardson A. Pharmacol Ther. 2019 195:172-185. doi: 10.1016/j.pharmthera.2018.11.001.

2018

Age-related focal loss of contractile vascular smooth muscle cells in retinal arterioles is accelerated by caveolin-1 deficiency. Reagan AM, Gu X, Paudel S, Ashpole NM, Zalles M, Sonntag WE, Ungvari Z, Csiszar A, Otalora L, Freeman WM, Stout MB, Elliott MH. Neurobiol Aging. 2018 Jul 10;71:1-12. doi: 10.1016/j.neurobiolaging.2018.06.039.
Exposure to environmental enrichment attenuates addiction-like behavior and alters molecular effects of heroin self-administration in rats. Imperio CG, McFalls AJ, Hadad N, Blanco-Berdugo L, Masser DR, Colechio EM, Coffey AA, Bixler GV, Stanford DR, Vrana KE, Grigson PS, Freeman WM. Neuropharmacology. 2018 Jun 28;139:26-40. doi: 10.1016/j.neuropharm.2018.06.037.
Caloric restriction mitigates age-associated hippocampal differential CG and non-CG methylation. Hadad N, Unnikrishnan A, Jackson JA, Masser DR, Otalora L, Stanford DR, Richardson A, Freeman WM. Neurobiol Aging. 2018 Jul;67:53-66. doi: 10.1016/j.neurobiolaging.2018.03.009.
Revisiting the genomic hypomethylation hypothesis of aging. Unnikrishnan A, Hadad N, Masser DR, Jackson J, Freeman WM, Richardson A. Ann N Y Acad Sci. 2018 Apr;1418(1):69-79. doi: 10.1111/nyas.13533.
Expression of the purine biosynthetic enzyme phosphoribosyl formylglycinamidine synthase in neurons. Mangold CA, Yao PJ, Du M, Freeman WM, Benkovic SJ, Szpara ML. J Neurochem. 2018 Mar;144(6):723-735. doi: 10.1111/jnc.14304.
Insulin-like growth factor receptor signaling regulates working memory, mitochondrial metabolism, and amyloid-β uptake in astrocytes. Logan S, Pharaoh GA, Marlin MC, Masser DR, Matsuzaki S, Wronowski B, Yeganeh A, Parks EE, Premkumar P, Farley JA, Owen DB, Humphries KM, Kinter M, Freeman WM, Szweda LI, Van Remmen H, Sonntag WE. Mol Metab. 2018 Mar;9:141-155. doi: 10.1016/j.molmet.2018.01.013.
17α-estradiol acts through hypothalamic pro-opiomelanocortin expressing neurons to reduce feeding behavior. Steyn FJ, Ngo ST, Chen VP, Bailey-Downs LC, Xie TY, Ghadami M, Brimijoin S, Freeman WM, Rubinstein M, Low MJ, Stout MB. Aging Cell. 2018 Feb;17(1). doi: 10.1111/acel.12703.
Analysis of DNA modifications in aging research. Masser DR, Hadad N, Porter H, Stout MB, Unnikrishnan A, Stanford DR, Freeman WM. Geroscience. 2018 Feb;40(1):11-29. doi: 10.1007/s11357-018-0005-3.
A potential common role of the Jumonji C domain-containing 1A histone demethylase and chromatin remodeler ATRX in promoting colon cancer. Li X, Oh S, Song H, Shin S, Zhang B, Freeman WM, Janknecht R.Oncol Lett. 2018 Nov;16(5):6652-6662. doi: 10.3892/ol.2018.9487.

2017

Sexually divergent DNA methylation patterns with hippocampal aging. Masser DR, Hadad N, Porter HL, Mangold CA, Unnikrishnan A, Ford MM, Giles CB, Georgescu C, Dozmorov MG, Wren JD, Richardson A, Stanford DR, Freeman WM. Aging Cell. 2017 Dec;16(6):1342-1352. doi: 10.1111/acel.12681.
Functional changes in the neural retina occur in the absence of mitochondrial dysfunction in a rodent model of diabetic retinopathy. Masser DR, Otalora L, Clark NW, Kinter MT, Elliott MH, Freeman WM. J Neurochem. 2017 Dec;143(5):595-608. doi: 10.1111/jnc.14216
Retinal gene expression responses to aging are sexually divergent. Du M, Mangold CA, Bixler GV, Brucklacher RM, Masser DR, Stout MB, Elliott MH, Freeman WM. Mol Vis. 2017 Oct 10;23:707-717
Sexually divergent induction of microglial-associated neuroinflammation with hippocampal aging. Mangold CA, Wronowski B, Du M, Masser DR, Hadad N, Bixler GV, Brucklacher RM, Ford MM, Sonntag WE, Freeman WM. J Neuroinflammation. 2017 Jul 21;14(1):141. doi: 10.1186/s12974-017-0920-8.
Role of DNA methylation in the dietary restriction mediated cellular memory. Unnikrishnan A, Jackson J, Matyi SA, Hadad N, Wronowski B, Georgescu C, Garrett KP, Wren JD, Freeman WM, Richardson A. Geroscience. 2017 Jun;39(3):331-345. doi: 10.1007/s11357-017-9976-8.
CNS-wide Sexually Dimorphic Induction of the Major Histocompatibility Complex 1 Pathway With Aging. Mangold CA, Masser DR, Stanford DR, Bixler GV, Pisupati A, Giles CB, Wren JD, Ford MM, Sonntag WE, Freeman WM. J Gerontol A Biol Sci Med Sci. 2017 Jan;72(1):16-29.
Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation. Hopiavuori BR, Masser DR, Wilkerson JL, Brush RS, Mandal NA, Anderson RE, Freeman WM. Methods Mol Biol. 2017;1609:33-41. doi: 10.1007/978-1-4939-6996-8_4.

2016

Loss of the antioxidant enzyme CuZnSOD (Sod1) mimics an age-related increase in absolute mitochondrial DNA copy number in the skeletal muscle. Masser DR, Clark NW, Van Remmen H, Freeman WM. Age. 2016 Aug;38(4):323-333. doi: 10.1007/s11357-016-9930-1.
Bisulfite oligonucleotide-capture sequencing for targeted base- and strand-specific absolute 5-methylcytosine quantitation. Masser DR, Stanford DR, Hadad N, Giles CB, Wren JD, Sonntag WE, Richardson A, Freeman WM. Age. 2016 Jun;38(3):49. doi: 10.1007/s11357-016-9914-1.
Assessment of individual differences in the rat nucleus accumbens transcriptome following taste-heroin extended access. Imperio CG, McFalls AJ, Colechio EM, Masser DR, Vrana KE, Grigson PS, Freeman WM. Brain Res Bull. 2016 May;123:71-80. doi: 10.1016/j.brainresbull.2015.12.005.
Reward devaluation and heroin escalation is associated with differential expression of CRF signaling genes. McFalls AJ, Imperio CG, Bixler G, Freeman WM, Grigson PS, Vrana KE. Brain Res Bull. 2016 May;123:81-93. doi: 10.1016/j.brainresbull.2015.11.009
Recent Developments in Understanding Brain Aging: Implications for Alzheimer’s Disease and Vascular Cognitive Impairment. Deak F, Freeman WM, Ungvari Z, Csiszar A, Sonntag WE. J Gerontol A Biol Sci Med Sci. 2016 Jan;71(1):13-20. doi: 10.1093/gerona/glv206
Absence of genomic hypomethylation or regulation of cytosine-modifying enzymes with aging in male and female mice. Hadad N, Masser DR, Logan S, Wronowski B, Mangold CA, Clark N, Otalora L, Unnikrishnan A, Ford MM, Giles CB, Wren JD, Richardson A, Sonntag WE, Stanford DR, Freeman W. Epigenetics Chromatin. 2016 Jul 13;9:30. doi: 10.1186/s13072-016-0080-6